Cyanide-Nitroprusside Colorimetric Assay: A Rapid Colorimetric Screen for Urinary Cystine

A Rapid Colorimetric Method for the Estimation of Urinary Chlorides.

A rapid and reproducible method for chloride estimation in urine is described. The colour produced is stable and obeys Beer's law over a wide range of concentrations using a simple colorimeter and filter. The method gives good agreement with both the potentiometric titration method of Sanderson (1952) and the mercurimetric titration method of Schales and Schales (1941). The precision of the col...

Rapid colorimetric assay for antimicrobial susceptibility testing of Pseudomonas aeruginosa.

A colorimetric assay based on the reduction of a tetrazolium salt [2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT)] for rapidly determining the susceptibility of Pseudomonas aeruginosa isolates to bactericidal antibiotics is described. There was excellent agreement between the tobramycin and ofloxacin MICs determined after 5 h using the XTT assay and after 18 h u...

Colorimetric Assay for Antifungal Susceptibility

Rapid microdilution-colorimetric assay for yeast susceptibility to fluorocytosine.

Acid production by certain yeast species through the fermentation of glucose was used as the basis of an in vitro test for measuring susceptibility of these organisms to 5-fluorocytosine. Serial dilutions of 5-fluorocytosine in yeast nitrogen base broth, with bromothymol blue indicator dye, were made on microtiter plates. A fixed-concentration suspension of yeast cells was added to successive w...

Rapid colorimetric assay for acetaminophen without salicylate or phenylephrine interference.

A simple rapid, and economical procedure is presented for the quantitative determination of acetaminophen in serum. Acetaminophen is extracted into solvent, and, when back-extracted into base, is simultaneously reacted with Folin-Ciocalteau reagent to form a colored complex. The potential interference of bilirubin is eliminated by treatment with p-diazobenzene sulfonic acid. Results obtained wi...